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Biophysical techniques

 

  • Malvern Zetasizer Nano ZS90: A device for the measurement of the hydrodynamic radii, zeta potential and electrophoretic mobility of molecules, and for the determination of the aggregation state of proteins under different conditions. Technique used: dynamic light scattering.
  • Chirascan Plus CD spectrometer: A spectrometer to measure circular dichroism spectra and absorbance as function of temperature, pH and concentration to determine the proportion and type of secondary structure present in protein samples, in peptides, conformation of RNA and DNA, as well as to detect conformational changes.
  • Microcal iTC200: A highly sensitive, low volume titration calorimeter for label-free solution studies of biomolecular interactions.
  • Malvern Microcal VP-DSC: Highly sensitive differential scanning calorimeter allowing the direct measurement of intramolecular stability of biological macromolecules, as well as the intermolecular stability of biologically-relevant complexes such as oligomeric proteins, nucleic acid duplexes, and micellar systems (lipid and detergent micelles).
  • Nano Temper Monolith NT.115: Microscale thermophoresis (MST) to study biomolecular interactions. The device allows to characterize protein-protein and protein-ligand (small molecule, DNA, RNA, peptides, sugars, lipids…) interactions, that can be measured under close to native conditions based on thermophoretic effect. Protein labeling is required with this device.
  • Nano Temper NT.LabelFree: Microscale thermophoresis (MST) device. Characterization of protein-ligand interactions based on thermophoretic effect, using the intrinsic tryptophan fluorescence. No sample modification is required with this device.
  • Nano Temper Prometheus NT.48: Measurement of protein stability using tyrosine and tryptophan fluorescence.
  • BioRad ProteOn XPR36: Label-free quantitative analysis of biomolecular interactions by the technique of surface plasmon resonance (SPR).
  • JenaAnalytic Specord 50 Plus: Precise double-beam spectrophotometer for UV/Vis measurements including using a temperature ramp.

 

 

Crystallisation of proteins and nucleic acids, Diffraction techniques

 

  • Spectrolight 600: In drop dynamic light scattering measurements in Terasaki 72-well plates.
  • ArtRobbins Gryphon dropsetter: A multi-channel (96 channels) pipetting robot for the easy set-up of nanodrop crystallisation plates.
  • Formulatrix RI1000 crystallisation hotel: A crystallisation plate storage and automated crystallization monitoring enclosure allowing remote access to crystallization images. Crystallisation drop images can be taken using visible light, polarized light and UV.
  • Glovebox with stereomicroscope for crystallization and crystal manipulation under defined (oxygen-free) atmosphere.
  • Bruker D8 Venture: diffractometer with a high-flux liquid Gallium X-ray source MetalJet D2, Photon II detector and Kappa goniometer or ISX stage. The diffractometer is used (at CMS) for X-ray diffraction of biomolecular crystals.
  • ISX stage for D8 Venture: motorized stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallization trays.

 

 

Structural Mass Spectrometry

 

  • Bruker Daltonics 15T-Solarix XR FT-ICR mass spectrometer, with electrospray and MALDI ion sources: This ultra-high resolution mass spectrometer is used mostly for the determination of the precise mass of biological macromolecules, and the characterization of their posttranslational modifications. Further possibilities include peptide mass fingerprinting, detection of small molecule/metabolites, monitoring of protein structural changes and protein-protein interactions under physiological conditions by hydrogen-deuterium exchange and chemical cross-linking.
  • Agilent Technologies 1200 HPLC system (usually coupled to the 15T-SolariX XR mass spectrometer) for the separation of complex peptide mixtures, proteins and metabolites.