The Institute of Biotechnology of the Czech Academy of Sciences announces in accordance with the Act No. 283/1992 Coll. on the Czech Academy of Sciences, and the Statutes of the Czech Academy of Sciences open full time position:
Postdoctoral fellow of a research project “Monitoring of protein network dynamics during sperm-egg membrane interaction”
Postdoctoral fellow will mainly be responsible for genomic and proteomic methods involved in the project, working with male and female gametes in mouse model, gamete maturation, interaction, in vitro fertilization, IF, IHC, WB, IP, microscopy, including super-resolution microscopy, production and purification of recombinant proteins and monoclonal antibodies, cell cultures, gene expression, PCR, qRT-PCR, data analysis and preparation for the manuscript, active part in writing publications and presenting the data.
- PhD in relevant field of Reproductive and Cell Biology
- Proved history of research activities
- Publication activity in the field
- Ability to actively participate in a research program
- Excellent communication skills and teamwork
- English, written and spoken, advanced level
What we offer
- The contract is offered for three years with a possible extension.
Start date: February, 2018
Workplace location: Centre of Excellence BIOCEV, Vestec, Prague West region, Czech Republic.
IBT is located in the BIOCEV Centre completed in the year 2015 and fully equipped for experimental work in molecular and cellular biology.
Send written applications including cover letter, professional CV and recommendation letter in electronic form to firstname.lastname@example.org
Application deadline: January 29, 2018
PhD and MSc students
Laboratory of Gene Expression is looking for two PhD and MSc candidates with an expected starting date for summer/autumn 2018.
Students will participate on projects focused on molecular and developmental biology.
Laboratory: The Laboratory of Gene Expression is a well-funded research laboratory with an excellent publication track record and comprise of researchers with unique scientific expertise. We focus on gene expression studies using a combination of up-to date techniques such as single cell expression profiling and NGS. We also utilize good biological models to help explain aspects of embryonic development, neurodegenerative diseases and cancer progression. The laboratory is located in the new state-of-the-art BIOCEV research center.
Qualification: Applicants should have a background in biochemistry, physiology, molecular or cell biology and bioinformatics. We seek a highly motivated, creative person, with strong interest in solving scientific problems.
- MicroRNA in central nervous system injury: potential roles and therapeutic implications. To understand the mechanisms underlying gene alterations following spinal cord injury and ischemic stroke, the successful candidate will examine temporal expression of miRNAs and mRNAs in the nervous tissue in mice and rat models using RNA sequencing and RT-qPCR analysis. The most promising miRNA-mRNA regulatory networks will be validated in vitro and in vivo using combination of tools for RNA, protein and functional analysis to verify true biological relevance of the findings. We are looking for candidate especially interested in large-scale data measurement and analysis.
- In the second project we use frog animal model to study early development and biological processes such as wound healing and regeneration. Student is expected to perform a broad set of experiments from functional experiments including microinjections and micromanipulations of embryos, gene expression characterization to large-scale data measurement and analysis.
Contact: For application or further information please contact Dr. Radek Šindelka or Dr. Lukáš Valihrach:
Laboratoř strukturních proteinů Biotechnologického ústavu AV ČR, v. v. i. hledá pracovníka/pracovnici na pozici
technický pracovník (úvazek 0,5)
- Příprava roztoků, pufrů, médií, mikroskopických skel
- Klonování, exprese a purifikace proteinů
- Administrativní práce v rámci laboratoře
- Minimálně ukončené středoškolské vzdělání s maturitou biochemického nebo příbuzného zaměření (praxe výhodou)
- Pečlivost, spolehlivost, samostatnost, zájem o problematiku, flexibilita
- Úvazek 0,5
- 5 týdnů dovolené
- Podpora dalšího vzdělávání
Nástup možný ihned.
Zdeněk Lánský, PhD
Biotechnologický ústav AV ČR, v. v. i.
252 50 Vestec
Tel: 325 873 772
PhD student position
Molecular mechanism of mitochondrial transfer in cancer
Background: In our previous work we established that cells devoid of mitochondrial DNA cannot form tumors in mice, unless they acquire mitochondrial DNA by the transfer of whole mitochondria from the stroma of the host animal (Tan et al. Mitochondrial genome acquisition restores respiratory function and tumorigenic potential in cancer cells without mitochondrial DNA. Cell Metab 2015, 21, 81-94; Dong et al. Horizontal transfer of whole mitochondria recovers tumorigenic potential in mtDNA-deficient cells. eLife 2017, 6, e22187). This suggests that functional mitochondria are essential for tumorigenesis and indicates that cancer cells have the means to ‘steal’ mitochondria from the surrounding stroma.
Project: In the current project, we will characterise more closely the mechanism by which this mitochondrial theft occurs. In particular, we will evaluate the role of the mitochondrial motor-adaptor complex (Miro/Milton/kinesin) by a combination of in vitro and in vivo approaches. Using genetic modifications, we will characterise the transfer of mitochondria via tunnelling nanotubes between cells in vitro and develop a mouse model (Miro1 conditional knockout mouse) to evaluate this process in vivo.
Laboratory: Laboratory of Molecular Therapy, headed by Prof. Jiri Neuzil, is an established research laboratory with excellent publication track record. We focus on mitochondria in the context of cancer, and on devising strategies to eliminate specific subsets of cancer cells by mitochondrial targeting. We are located in the new state- of-the-art BIOCEV research centre.
Qualification: Applicants should have background in biochemistry, physiology, molecular or cell biology. We seek a highly motivated, creative person, with strong interest in solving scientific problems.
Contact: For more information please contact Jakub Rohlena - email@example.com or Jiri Neuzil - firstname.lastname@example.org
Open position at the Centre of Molecular Structure
Specialist in biological X-ray techniques, especially small-angle X-ray scattering (SAXS) and single crystal X-ray diffraction (plus crystallization), full-time
The Centre of Molecular Structure (CMS), part of CIISB (the Czech Infrastructure for Integrative Structural Biology) and one of the European network ERIC-Instruct sites, is operated by the Institute of Biotechnology CAS v. v. i. (BIOCEV), Vestec, central Bohemia, Czech Republic). The CMS is a technology platform providing access to scientific instrumentation and service to researchers, mostly working in “life sciences”. Details of the scientific equipment and services provided at the CMS can be consulted on this link.
We are looking for a specialist in X-ray techniques. The focus of the position is to provide excellent service in maintaining and operating a newly acquired state-of-the-art SAXS line and partially also the X-ray diffraction and crystallisation platform. User support providing training, assistance or sometimes full service (depending on the particular case) is also expected.
The successful candidate will also have the opportunity to collaborate in exciting research projects involving X-rays (both diffraction and scattering) in IBT research groups focused on structural biology. For such additional duties (that will represent up to 50% of the time), practical knowledge of protein and nucleic acid purification and crystallisation is a plus. Participation to data collection trips at synchrotron radiation beam lines is expected.
The applicants (preferably having Ph.D. level experience) must have practical experience with bio-SAXS and have a strong interest in providing training and assistance to users and students.
The position offered is for an initial period of three years, with the possibility of renewal.
Applications (in electronic format, Word document or pdf files preferred) including a cover letter, curriculum vitae, and the names and addresses of three referees must be sent to the main office of the Institute of Biotechnology:
Institute of Biotechnology CAS, v. v. i.
252 50 Vestec
Phone: +420 325 873 700
Any questions regarding this position can be directed to
Dr Frederic Vellieux, email@example.com
or Dr Jan Dohnalek, firstname.lastname@example.org
Application deadline: 15 December 2017
Expected starting date: within the period January – March 2018
Laboratoř inženýrství vazebných proteinů hledá
motivované Bc. studenty
pro práci v biochemicky orientované laboratoři s možností vypracování bakalářské práce v rámci jednoho ze dvou řešených projektů:
- Immunomodulatory ligands targeted to IL-23/Th17 pro-inflammatory axis as novel tools for development of topical drugs for treatment of psoriasis
- Construction of recombinant mimotopes for induction of neutralizing antibodies against HIV-1 gp120 glycoprotein using high-affinity binders approach
Pro studenta bude v případě zájmu k dispozici částečný pracovní úvazek.
Informace o laboratoři zde.
Do našeho týmu hledáme zkušeného laboratorního pracovníka na plný úvazek pro servisní laboratoř GeneCore Biotechnologického ústavu AV ČR, v. v. i. v BIOCEVu, která je předním evropským pracovištěm nabízející qPCR a NGS služby.
laboratorní pracovník na plný úvazek
The laboratory worker will work on client’s projects in molecular analysis mainly with real-time PCR and next generation sequencing technologies. The work encompasses all aspects of workflow from sampling, extraction, quality control, molecular analysis, and data analysis. The laboratory worker also contributes as instructor in hands-on training courses in molecular analyses. The laboratory worker is expected to work independently on projects delegated by the Gene Core manager. The GeneCore facility primarily serves researchers at the BIOCEV campus, but also other researcher in Czech Republic and occasional also international customers. The GeneCore collaborates close with TATAA Biocenter (www.tataa.com).
- Vysokoškolské vzdělání příslušného směru s odbornou praxí
- Zájem o technologie a metody pro molekulární analýzy
- Schopnost pracovat jak samostatně tak i ve skupině
- Spolehlivost a flexibilita
- Chuť do práce a odhodlání učit se novým věcem
- Znalost anglického jazyka slovem i písmem
- Pracovní poměr na dobu určitou s možností prodloužení (možné i na zkrácený úvazek)
- Odpovídající platové ohodnocení
- Atraktivní pracovní prostředí moderního vědeckého centra
- Přátelský mezinárodní kolektiv
- Pět týdnů dovolené
- Příspěvek na stravování
Žádost prosím pošlete e-mailem na adresu: Veronika.Kasparova@ibt.cas.cz
Datum nástupu do zaměstnání: dle dohody v průběhu podzimu 2017.
Pracoviště: GeneCore BIOCEV, Průmyslová 595, 252 50 Vestec, Česká republika
PhD student position
The role of protein friction in the dynamics of cytoskeletal filaments
The cytoskeleton is the intracellular network of dynamical filaments, interconnected and regulated by associated proteins, that both generates intracellular mechanical forces and withstands forces from the outside. One of the canonical force-generating mechanism in cytoskeleton is the assembly and disassembly of cytoskeletal filaments, termed the filament dynamics. Friction limits the force generation in all mechanical systems, but experimental studies of its effect on the mechanics of cytoskeleton have only just begun. How friction affects filament dynamics, remains an open question.
Project: The project, based on previous results (Lansky, Braun et al. Cell 2015, 160(6): 1159-68 and Braun, Lansky et al. Nat Cell Biol 2011, 13(10): 1259-64), will be focused on quantitative characterization of the effects of friction-generating cytoskeletal proteins on dynamics of cytoskeletal filaments. The individual proteins will be expressed in a recombinant form, combined with cytoskeletal filaments in vitro and the emergent system will be investigated using a combination of advanced biophysical and biochemical experimental techniques such as single molecule fluorescence microscopy and optical tweezers.
Candidate's profile: Eligible candidates should have a degree in (bio)physics, biology, chemistry or equivalent and should be interested in cross-disciplinary experimental work.
Marcus Braun, PhD
Institute of Biotechnology CAS, v. v. i.
Průmyslová 595, 252 50 Vestec