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Vědecká pracoviště

The Institute of Biotechnology of the Czech Academy of Sciences (IBT) announces in accordance with the Act no. 283/1992 Coll. on the Academy of Sciences of the Czech Republic, as amended by Act no. 420/2005 Coll. and the Statutes of the CAS an open full-time position

 

Leader of a research project “Dynamics of Biological Processes”

 

Requirements:
  • PhD in biophysics, physics, physical chemistry, or biochemistry
  • Publication activity in the field
  • Proven ability to lead a research group and formulate its research program
  • Ability to get funding (national or international programs)
  • Excellent communication skills and teamwork
  • Readiness for high work load
  • English, written and spoken, advanced level
  • Good repute

 

Job description:
The successful candidate will have a genuine interest in understanding of the structure and dynamics of nucleic acids and proteins. He or she will design, coordinate and execute experiments aimed at measuring (meresolved spectroscopies oriented on resolving biomolecular dynamics. Projects will be formulated by the candidate but the preferred experimental approaches include time resolved transient absorption, time resolved vibration spectroscopy such as femtosecond infrared and/or Raman spectroscopy, multidimensional vibration and electronic spectroscopy, and time resolved X-ray diffraction. Efficient combination of complementary techniques is welcome.
The successful candidate will work at IBT and collaborate with the Institute’s research groups. However, time resolved experiments will be designed and executed at ELI-Beamlines in close collaboration with the ELI teams led by Prof. Janos Hajdu and Dr. Jakob Andreasson.
The contract is offered for three years with a possible extension.
 

Start date possible immediately or as soon as possible
 

Workplace location: Centre BIOCEV, Vestec, and ELI-Beamlines, Dolni Brezany, both Prague West region, Czech Republic.
 

IBT is located at the BIOCEV Centre completed in the year 2015 and fully equipped for experimental work in molecular and cellular biology with emphasis on structural and biophysical characterization of studied molecular systems. ELI-Beamlines is located about 5 km from BIOCEV and offers opportunity for breakthrough measurements of time-resolved molecular properties using radiation from THz frequencies to X-rays with up to femtosecond resolution. A close collaboration between teams from IBT and ELI-Beamlines and their physical closeness opens up a unique opportunity to perform medically and biotechnologically oriented projects by using technologies of structural biology and photon physics not available elsewhere.
 

Send written applications including a cover letter and a professional CV, either in printed or electronic form to address:
Institute of Biotechnology CAS, v. v. i., Prumyslova 595, 25250 Vestec, Czech Republic
bohdan.schneider@ibt.cas.cz

                                                                                                                

PhD student position

 

Project title: Development and maintenance of Beta cells in the pancreas

 

Project description: This project will focus on molecular mechanisms underlying development, maturation and function of endocrine beta cells in the pancreas. This project will aim at identifying biomarkers associated with glycemic deterioration in pre-diabetes to diabetes, and diabetes progression via Beta cell dysfunction. The methods used will include knockouts using Cre/loxP and Crispr/Cas9 technologies, global gene expression analyses (RNAseq), immunohistochemistry, and single cell analysis (Beta-cells) of selected targets.

 

Candidate’s requirements:

M.Sc. or equivalent in cell, molecular and/or developmental biology.

 

Contact: For application or further information please contact Gabriela Pavlinkova: gpavlinkova@ibt.cas.cz

                                                                                                                

Biotechnologický ústav AV ČR, v. v. i. a Ústav experimentální medicíny AV ČR, v. v. i., divize BIOCEV, hledají uchazečky či uchazeče pro pozici

 

laborantky/a pro společné pracoviště BTÚ/ÚEM v centru BIOCEV ve Vestci u Prahy

 

Místo výkonu práce: centrum BIOCEV ve Vestci u Prahy

Termín nástupu: ihned nebo dle dohody

 

Náplň práce:

- objednávky zboží a související evidence, přebírání a ukládání zásilek společného pracoviště BTÚ/ÚEM v centru BIOCEV

- základní kultivační práce s buněčnými kulturami

- materiální zabezpečení chodu laboratoře (pravidelné objednávky, sklo, evidence chemikálií, sanitace apod.)

- zkušenost z extrakce RNA/DNA, PCR, elektroforézou, imunohistochemií, práce s buňkami výhodou

 

Požadujeme:

- minimálně středoškolské vzdělání s maturitou

- ochota pracovat na výzkumném pracovišti

- komunikační schopnosti, flexibilita, schopnost a ochota se naučit novým metodikám a pečlivost

- znalost práce se standardním kancelářským softwarem

- praxi v používání laboratorních přístrojů a software výhodou

- schopnost komunikovat v anglickém jazyce výhodou

- zkušenosti na podobné pozici z prostředí VVI nebo VŠ výhodou

 

Nabízíme:

- pracovní poměr na dobu určitou s možností prodloužení (možné i na zkrácený úvazek)

- odpovídající platové ohodnocení

- atraktivní pracovní prostředí moderního vědeckého centra

- přátelský mezinárodní kolektiv

- pět týdnů dovolené

- příspěvek na stravování

 

Přihlášky do výběrového řízení s krátkým motivačním dopisem a strukturovaným CV prosím zasílejte pouze elektronicky na email romanyuk@biomed.cas.cz

                                                                                                                

Laboratory technician

 

The Centre of Molecular Structure (CF Crystallization of proteins and nucleic acids and CF Biophysical techniques) of the Institute of Biotechnology CAS, v. v. i. at BIOCEV, Vestec, is looking for a laboratory technician for a full time position

 

Job description: The laboratory technician will participate in the work required by the crystallization facility (preparing solutions, transferring and pipetting, measurements of pH, filtering etc) together with additional duties in the section dealing with biophysical measurements. As a Core Facility serving the needs of a community of researchers mainly in the Czech Republic, emphasis is placed on the quality of the service offered. The work performed by the technician will be carried out under the supervision of the scientists in charge of the respective services, as well as under the supervision of the Core Facility head.
 

Requirements:

  • Master’s degree (M.Sc.) in an appropriate field related to day-to-day work in a research laboratory (Chemistry, Biology, Biochemistry) with demonstrated experience in laboratory work. Experience in crystallization and operating biophysical measurement instruments preferred.
  • Ability to perform the duties independently, with little supervision
  • Ability to work in English (reading, writing and speaking English)

 

The contract is offered for 3 years.


Start date: as soon as possible


Workplace: Centre of Excellence BIOCEV, Vestec, Prague region, Czech Republic

 

Send written applications (in English) including cover letter, curriculum vitae and letter of recommendation in electronic form (Word or PDF documents) to frederic.vellieux@ibt.cas.cz

 

Application deadline: 28. 2. 2017

                                                                                                                

PhD student position

 

The role of protein friction in the dynamics of cytoskeletal filaments

 

The cytoskeleton is the intracellular network of dynamical filaments, interconnected and regulated by associated proteins, that both generates intracellular mechanical forces and withstands forces from the outside. One of the canonical force-generating mechanism in cytoskeleton is the assembly and disassembly of cytoskeletal filaments, termed the filament dynamics. Friction limits the force generation in all mechanical systems, but experimental studies of its effect on the mechanics of cytoskeleton have only just begun. How friction affects filament dynamics, remains an open question.2017_student_flyer.png
 

Project: The project, based on previous results (Lansky, Braun et al. Cell 2015, 160(6): 1159-68 and Braun, Lansky et al. Nat Cell Biol 2011, 13(10): 1259-64), will be focused on quantitative characterization of the effects of friction-generating cytoskeletal proteins on dynamics of cytoskeletal filaments. The individual proteins will be expressed in a recombinant form, combined with cytoskeletal filaments in vitro and the emergent system will be investigated using a combination of advanced biophysical and biochemical experimental techniques such as single molecule fluorescence microscopy and optical tweezers.  


Candidate's profile: Eligible candidates should have a degree in (bio)physics, biology, chemistry or equivalent and should be interested in cross-disciplinary experimental work.
 

Contact: For application or further information please contact Marcus Braun or Zdeněk Lánský:
braun@mpi-cbg.de
zdenek.lansky@ibt.cas.cz
 

Marcus Braun, PhD
BIOCEV
Institute of Biotechnology CAS, v. v. i.
Průmyslová 595, 252 50 Vestec
Czech Republic
www.biocev.eu

                                                                                                                

PhD student position

 

Physiological functions of GCPII orthologs in basal organisms

 

Summary: Human glutamate carboxypeptidase II (GCPII) is a zinc-dependent peptidase that is increasingly recognized as a target of therapeutic interventions in a variety of neurologic disorders as well as a marker for imaging and therapy of prostate cancer. However, despite of nearly universal expression of GCPII-like protein in living organism there is limited knowledge of physiological function(s) of its orthologs in non-mammalian species.
 

Project: The project focuses on structure-function studies of non-mammalian orthologs of human GCPII, including enzymes from C.elegans, F.hepatica, D.rerio, A. thaliana, S.mansoni and yeast species. Used methodology includes cloning strategies (Gateway), mutagenesis, heterologous protein expression in various systems (E.coli, K.lactis, insect and mammalian cells), protein purification and characterization, enzymatic assays including high-throughput library screening, inhibition studies, cell biology (fluorescent microscopy), and X-ray crystallography. We believe that unravelling physiological function(s) of these proteins in non-mammalian species could shed light unto yet unknown functions of the protein in mammals, including humans.
 

Qualifications: Applicants should have a solid background in molecular biology and biochemistry or cell biology. We expect good communication skills, analytical thinking and the ability for teamwork. The successful candidate will participate in a PhD program at Charles University in Prague. The starting date is summer/fall 2016.

 

How to Apply: For more information please contact Cyril Bařinka (cyril.barinka@ibt.cas.cz) directly.

 

Cyril Barinka, PhD
Laboratory of Structural Biology
BIOCEV, Centre of Excellence
25242 Vestec u Prahy
http://academy5.avcr.cz/lsb/

                                                                                                                

PhD student position

 

Molecular basis of substrate recognition by histone deacetylases (HDACs)

 

Summary: Protein acetylation has emerged as a key regulatory mechanism of fundamental cellular processes in all living organisms. However, there is a limited understanding of structural and functional features of the enzymatic machinery (histone acetyltransferase and histone deacetylases) implicated in these processes. Histone deacetylase 6 (HDAC6) is a unique member of the HDAC family and is an attractive target for therapeutic interventions in cancers and neuropathologies. Several HDAC6 substrates have been identified up to date, yet our understanding of HDAC6 cellular functions is quite limited.


Project: The project focuses on unraveling the molecular basis of the substrate recognition by HDAC6. We have successfully established a methodology for heterologous production of HDAC6 variants in a mammalian expression system. A set of molecular biochemical and biophysical approaches will be harnessed to define general principles governing substrate recognition by HDAC6.


Job description: We are seeking a highly motivated PhD candidate to join our research team investigating a structure and function of human HDAC6 and related enzymes. We are using methods of molecular biology (cloning, mutagenesis, heterologous expression), biochemistry (protein purification, enzymatic assays), and biophysics (surface plasmon resonance, thermophoresis, X-ray crystallography, electron microscopy) to unravel fundamental molecular characteristics of HDAC6 and related deacetylases.


Qualifications: Applicants should have a solid background in molecular biology and biochemistry or cell biology. We expect good communication skills, analytical thinking and the ability for teamwork. The successful candidate will participate in a PhD program at Charles University in Prague. The starting date is summer/fall 2016.


How to Apply: For more information please contact Cyril Bařinka (cyril.barinka@ibt.cas.cz) directly.

 

Cyril Barinka, PhD
Laboratory of Structural Biology
BIOCEV, Centre of Excellence
25242 Vestec u Prahy
http://academy5.avcr.cz/lsb/