Laboratory of Biomolecular Recognition

We study interactions driving specific recognition between biomolecules with a potential diagnostic, medical, or biotechnological use.

Group leader: prof. Ing. Bohdan Schneider, CSc., DSc.

Detailed laboratory website: https://biorecognition.structbio.org/index.html

Our goal is to understand how biological functions relate to structures of biomolecules and their interactions. We therefore concentrate on determination of biomolecular structures and dynamics of their behavior and study specific protein-protein and protein-DNA interactions, their mutual recognition. In our work, we take advantage of experimental and computational methods of protein engineering, structural biology, bionformatics, and molecular modeling.

In the lab, we run several projects:

  • Cytokines. We study structure-function relationships of cytokines from the family of Interleukin 10. These signaling proteins and their receptors are important in the immunity response to viral and bacterial infection, and errors in their regulation cause serious autoimmune and/or allergic health disorders and may even promote malignancy. We concentrate on structural aspects of the cytokine interactions, determine their crystal structures, and modify their molecules to make them more stable. We also apply methods of directed evolution (ribosome and yeast display) to develop de novo proteins binding specifically FIL-10 cytokines or their receptors.
  • Bioinformatics. The lab concentrates on structural analysis of nucleic acids, DNA and RNA, and on hydration around proteins and nucleic acids.

Nucleic acids are structurally plastic molecules. To identify their structural polymorphisms we analyzed structures of dinucleotide building blocks in thousands of crystal structures. We clustered them into so called conformational classes NtC and so called structural alphabet CANA. The work is summarized in several publications and the results are available on the website dnatco.org.

Hydration. Water molecules in the first hydration shell of amino acids residues in proteins and dinucleotides in DNA are well ordered and the structure of the shell depends on the biomolecular structure and its sequence.

  • Single stranded DNA (ssDNA). ssDNA is known to play important yet largely unknown functions in genome regulation. We study one specific class of bacterial ssDNA, so called Repetitive extragenic palindromes (REP) and their associated enzyme, tyrosine transposase (RAYT).
  • Dynamics of Biomolecules. In collaboration with colleagues at ELI-Beamlines, we explore new frontiers in light and optics to create breakthrough science about biomolecular dynamics from femtoseconds to seconds.
Collaborations. The lab is a part of the Institute of Biotechnology of the Czech Academy of Sciences located at the BIOCEV center. It participates in the research program "Structural Biology and Protein Engineering”. We are also active in Instruct-ERIC and ELIXIR (3D-Bioinfo community) infrastructural projects.

 

Andersson Inger Agneta
Andrikopoulos Prokopios
Berdár Daniel
Biedermannová Lada, RNDr., Ph.D.
Fuertes Vives Gustavo
Henclová Tereza, Ing.
Herynek Štěpán, Mgr.
Huličiak Maroš, Mgr.
Chaudhari Aditya Suresh
Kolářová Lucie, Ing.
Liu Yingliang
Nečasová Iva, Mgr.
Pham Phuong Ngoc, Mgr.
Svoboda Jakub
Škultétyová Ľubica, RNDr., Ph.D.
Tomala Jakub, RNDr., Ph.D.

2021

  • Phuong, N.P., Huličiak, M., Biedermannová, L.,  Černý, J., Charnavets T., Fuertes, G., Herynek, Š., Kolářová, L., Kolenko P.,  Pavlíček J., Zahradník, J., Mikulecky, P., Schneider, B. (2021)"Protein Binder (ProBi) as a New Class of Structurally Robust Non-Antibody Protein Scaffold for Directed Evolution Viruses 13(2), 190

2020

  • Andrikopoulos, P. C., Y. Liu, A. Picchiotti, N. Lenngren, M. Kloz, A. S. Chaudhari, M. Precek, M. Rebarz, J. Andreasson, J. Hajdu, B. Schneider and G. Fuertes (2020). "Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations." Phys Chem Chem Phys 22(12), 6538-6552.

  • Cerny, J., Bozikova, P., Maly, M., Tykac, M., Biedermannova, L., & Schneider, B. (2020). "Structural alphabets for conformational analysis of nucleic acids available at dnatco.datmos.org." Acta Crystallogr D Struct Biol, 76(Pt 9), 805-813.
  • Cerny, J., P. Bozikova, J. Svoboda and B. Schneider (2020). "A unified dinucleotide alphabet describing both RNA and DNA structures." Nucleic Acids Research.

  • Orengo, C., Velankar, S., Wodak, S., Zoete, V., Bonvin, A., Elofsson, A., Feenstra, K. A., Gerloff, D. L., Hamelryck, T., Hancock, J. M., Helmer-Citterich, M., Hospital, A., Orozco, M., Perrakis, A., Rarey, M., Soares, C., Sussman, J. L., Thornton, J. M., Tuffery, P., Tusnady, G., Wierenga, R., Salminen, T., & Schneider, B. (2020). "A community proposal to integrate structural bioinformatics activities in ELIXIR (3D-Bioinfo Community)." F1000Res, 9.
  • Pizl, M., Picchiotti, A., Rebarz, M., Lenngren, N., Yingliang, L., Zalis, S., Kloz, M., & Vlcek, A. (2020). "Time-Resolved Femtosecond Stimulated Raman Spectra and DFT Anharmonic Vibrational Analysis of an Electronically Excited Rhenium Photosensitizer." J Phys Chem A, 124(7), 1253-1265.

2019

  • Fuertes, G., L. Nevola and S. Esteban-Martín (2019). Perspectives on drug discovery strategies based on IDPs. Intrinsically Disordered Proteins: 275-327.

  • Zahradnik, J., L. Kolarova, Y. Peleg, P. Kolenko, S. Svidenska, T. Charnavets, T. Unger, J. L. Sussman and B. Schneider (2019). "Flexible regions govern promiscuous binding of IL-24 to receptors IL-20R1 and IL-22R1." FEBS J 286(19): 3858-3873.

2018

  • Fuertes, G., N. Banterle, K. M. Ruff, A. Chowdhury, R. V. Pappu, D. I. Svergun and E. A. Lemke (2018). "Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water"." Science 361(6405).

  • Palyzova, A., J. Zahradnik, H. Maresova, L. Sokolova, E. Kyslikova, M. Grulich, V. Stepanek, T. Rezanka and P. Kyslik (2018). "Potential of the strain Raoultella sp. KDF8 for removal of analgesics." Folia Microbiol (Praha) 63(3): 273-282.

  • Schneider, B., P. Boaeikova, I. Necasova, P. Cech, D. Svozil and J. Cerny (2018). "A DNA structural alphabet provides new insight into DNA flexibility." Acta Crystallogr D Struct Biol 74(Pt 1): 52-64.

  • Zahradnik, J., L. Kolarova, H. Parizkova, P. Kolenko and B. Schneider (2018). "Interferons type II and their receptors R1 and R2 in fish species: Evolution, structure, and function." Fish Shellfish Immunol 79: 140-152.

  • Zahradnik, J., P. Kolenko, A. Palyzova, J. Cerny, L. Kolarova, E. Kyslikova, H. Maresova, M. Grulich, J. Nunvar, M. Sulc, P. Kyslik and B. Schneider (2018). "The crystal structure of XdpB, the bacterial old yellow enzyme, in an FMN-free form." Plos One 13(4): e0195299.

  • Zahradnik, J., J. Nunvar, H. Parizkova, L. Kolarova, A. Palyzova, H. Maresova, M. Grulich, E. Kyslikova and P. Kyslik (2018). "Agrobacterium bohemicum sp. nov. isolated from poppy seed wastes in central Bohemia." Syst Appl Microbiol 41(3): 184-190.

2017

  • Cerny, J., B. Schneider and L. Biedermannova (2017). "WatAA: Atlas of Protein Hydration. Exploring synergies between data mining and ab initio calculations." Phys Chem Chem Phys 19(26): 17094-17102.

  • Schneider, B., P. Bozikova, P. Cech, D. Svozil and J. Cerny (2017). "A DNA Structural Alphabet Distinguishes Structural Features of DNA Bound to Regulatory Proteins and in the Nucleosome Core Particle." Genes (Basel) 8(10).

2016

  • Biedermannova, L. and B. Schneider (2016). "Hydration of proteins and nucleic acids: Advances in experiment and theory. A review." Biochim Biophys Acta 1860(9): 1821-1835.

  • Cerny, J., P. Bozikova and B. Schneider (2016). "DNATCO: assignment of DNA conformers at dnatco.org." Nucleic Acids Research 44(W1): W284-287.

  • Mikulecky, P., J. Zahradnik, P. Kolenko, J. Cerny, T. Charnavets, L. Kolarova, I. Necasova, P. N. Pham and B. Schneider (2016). "Crystal structure of human interferon-gamma receptor 2 reveals the structural basis for receptor specificity." Acta Crystallogr D Struct Biol 72(Pt 9): 1017-1025.

2015

  • Biedermannova, L. and B. Schneider (2015). "Structure of the ordered hydration of amino acids in proteins: analysis of crystal structures." Acta Crystallogr D Biol Crystallogr 71(Pt 11): 2192-2202.

  • Bockova, M., T. Springer, I. Necasova, J. Nunvar, B. Schneider and J. Homola (2015). "Monitoring RAYT activity by surface plasmon resonance biosensor." Anal Bioanal Chem 407(14): 3985-3993.

  • Cerny, J., L. Biedermannova, P. Mikulecky, J. Zahradnik, T. Charnavets, P. Sebo and B. Schneider (2015). "Redesigning protein cavities as a strategy for increasing affinity in protein-protein interaction: interferon- gamma receptor 1 as a model." Biomed Research International 2015: 716945.

  • Craveur, P., A. P. Joseph, J. Esque, T. J. Narwani, F. Noel, N. Shinada, M. Goguet, S. Leonard, P. Poulain, O. Bertrand, G. Faure, J. Rebehmed, A. Ghozlane, L. S. Swapna, R. M. Bhaskara, J. Barnoud, S. Teletchea, V. Jallu, J. Cerny, B. Schneider, C. Etchebest, N. Srinivasan, J. C. Gelly and A. G. de Brevern (2015). "Protein flexibility in the light of structural alphabets." Front Mol Biosci 2: 20.

  • Charnavets, T., J. Nunvar, I. Necasova, J. Volker, K. J. Breslauer and B. Schneider (2015). "Conformational diversity of single-stranded DNA from bacterial repetitive extragenic palindromes: Implications for the DNA recognition elements of transposases." Biopolymers 103(10): 585-596.

2014

  • Kuchar, M., L. Vankova, H. Petrokova, J. Cerny, R. Osicka, O. Pelak, H. Sipova, B. Schneider, J. Homola, P. Sebo, T. Kalina and P. Maly (2014). "Human interleukin-23 receptor antagonists derived from an albumin-binding domain scaffold inhibit IL-23-dependent ex vivo expansion of IL-17-producing T-cells." Proteins 82(6): 975-989.

  • Nunvar, J., D. Elhottova, A. Chronakova, B. Schneider and I. Licha (2014). "Draft Genome Sequence of Stenotrophomonas maltophilia Strain 5BA-I-2, a Soil Isolate and a Member of a Phylogenetically Basal Lineage." Genome Announc 2(2).

  • Schneider, B., J. Cerny, D. Svozil, P. Cech, J. C. Gelly and A. G. de Brevern (2014). "Bioinformatic analysis of the protein/DNA interface." Nucleic Acids Research 42(5): 3381-3394.

  • Schneider, B., J. C. Gelly, A. G. de Brevern and J. Cerny (2014). "Local dynamics of proteins and DNA evaluated from crystallographic B factors." Acta Crystallogr D Biol Crystallogr 70(Pt 9): 2413-2419.

2013

  • Cech, P., J. Kukal, J. Cerny, B. Schneider and D. Svozil (2013). "Automatic workflow for the classification of local DNA conformations." BMC Bioinformatics 14: 205.

  • Kratochvilova, I., M. Vala, M. Weiter, M. Sperova, B. Schneider, O. Pav, J. Sebera, I. Rosenberg and V. Sychrovsky (2013). "Charge transfer through DNA/DNA duplexes and DNA/RNA hybrids: complex theoretical and experimental studies." Biophys Chem 180-181: 127-134.

  • Mikulecky, P., J. Cerny, L. Biedermannova, H. Petrokova, M. Kuchar, J. Vondrasek, P. Maly, P. Sebo and B. Schneider (2013). "Increasing affinity of interferon-gamma receptor 1 to interferon-gamma by computer-aided design." Biomed Research International 2013: 752514.

  • Nunvar, J., I. Licha and B. Schneider (2013). "Evolution of REP diversity: a comparative study." Bmc Genomics 14: 385.

2011

  • Benda, L., B. Schneider and V. Sychrovsky (2011). "Calculating the response of NMR shielding tensor sigma(31P) and 2J(31P,13C) coupling constants in nucleic acid phosphate to coordination of the Mg2+ cation." J Phys Chem A 115(11): 2385-2395.

2010

  • Joseph, A. P., G. Agarwal, S. Mahajan, J. C. Gelly, L. S. Swapna, B. Offmann, F. Cadet, A. Bornot, M. Tyagi, H. Valadie, B. Schneider, C. Etchebest, N. Srinivasan and A. G. De Brevern (2010). "A short survey on protein blocks." Biophys Rev 2(3): 137-147.

  • Kratochvilova, I., T. Todorciuc, K. Kral, H. Nemec, M. Buncek, J. Sebera, S. Zalis, Z. Vokacova, V. Sychrovsky, L. Bednarova, P. Mojzes and B. Schneider (2010). "Charge transport in DNA oligonucleotides with various base-pairing patterns." J Phys Chem B 114(15): 5196-5205.

2009

  • Vokacova, Z., M. Budesinsky, I. Rosenberg, B. Schneider, J. Sponer and V. Sychrovsky (2009). "Structure and dynamics of the ApA, ApC, CpA, and CpC RNA dinucleoside monophosphates resolved with NMR scalar spin-spin couplings." J Phys Chem B 113(4): 1182-1191.

MEYS: LTAUSA18197, Schneider: Design, development, and testing of bioinformatic tools for validation of experimental and computer molecular models in structural biology, biotechnology and pharmacy. 2019-2022.

GACR: GA19-17398S, Schneider: De novo development of bivalent protein binders mimicking the function of interferons lambda. 2019-2021.

MEYS: LM2015047, Schneider: ELIXIR CZ: Czech national infrastructure for biological data. 2016-2019.

GACR: GA16-20507S, Schneider: Interleukins of the IL-10 family: specificity and targeted modulation of interactions with receptors. 2016-2018.

MEYS: CZ.1.07/2.3.00/30.0020, Schneider, Charnavets, Pavlínková, Dodd, Hejnalová: Biotechnological expert. 2012-2015.

GACR: GAP305/12/1801, Schneider: Molecular mechanisms of association of a novel type of transposase with repetitive palindromic elements. 2012-2014.

GACR: GPP205/12/P729, Biedermannová: Effect of protein hydration shell on the stability of protein complexes. 2012-2014.

GACR: GAP305/10/2184, Schneider: Structure-function relationships underlying protein-protein interactions. 2010-2014.

GACR: GAP302/10/0427, Vondrášek: Production and characterization of biologically active recombinant human ameloblastina hard tissue regeneration and differentiation inducing protein. 2010-2012.

MEYS: MEB021032, Schneider: Complex structural analysis of interaction at the protein – nucleic acid interface by unique bioinformatic descriptors. 2010-2011.