CF Biophysical Techniques
The core facility Biophysical techniques enables assessment of the quality, stability and interaction properties of hundreds of biomolecular samples of many structural biology projects, be it regular checks, thorough analysis of properties or optimization of molecular constructs or handling protocols.
CF Crystallization of Proteins and Nucleic Acids
The core facility Crystallization of proteins and nucleic acids enables thousands of crystallization experiments using robotic or manual setup, automated monitoring of crystal growth, experiments at selected temperatures or under defined conditions, to prepare samples for further crystallographic studies.
CF Diffraction techniques
The core facility devoted to diffraction analysis offers crystal quality screening, in situ crystal testing, single crystal data collection and processing, small angle X-ray scattering (SAXS) experiments with robotic sample loading and online UV-VIS spectrometry, and SAXS data processing. Hundreds of samples are processed per year in the self-assisted mode or with full staff support.
CF Structural Mass Spectrometry
The core facility Structural mass spectrometry provides analyses of hundreds of samples and supports many internal and external structural biology projects. The main focus of the services lies in monitoring of proteins structural changes and protein-protein interaction by chemical cross-linking and hydrogen-deuterium exchange.
CF Protein Production
The core facility for protein production provides comprehensive services, covering every step from DNA to purified protein. These include gene cloning into expression vectors using various techniques and heterologous expression in Escherichia coli for bacterial expression, HEK293T, Expi293, and Expi293F™ GnTI- for mammalian expression, and the baculoviral system in Sf9 or High Five™ insect cells. The expression is followed by multistep protein purification.
Acknowledgement
The Centre of Molecular Structure is financially supported by the MEYS project LM2018127 Czech Infrastructure for Integrative Structural Biology (CIISB), CIISB4HEALTH (CZ.02.1.01/0.0/0.0/16_013/0001776), UP CIISB (CZ.02.1.01/0.0/0.0/18_046/0015974), and ELIBIO (CZ.02.1.01/0.0/0.0/15_003/0000447)
