Preparation of plasmid DNA constructs
Input: Target plasmid, DNA encoding the target protein, or purchased gene from common suppliers.
Output: Finished plasmid DNA, sequencing results confirming cloning success.
Time: Approx. 2 weeks
Approximate price: From 21 000 (cloning of 1 construct in one step) to 40 000 (multistep cloning of 1 construct)
EExpression of protein constructs in prokaryotic expression systems
Description: Various E.coli strains, expression optimization, bulk expression.
Input: Expression plasmid.
Output: Bacterial pellets containing the desired protein. Optimized expression conditions.
Time: Several days to a week
Approximate price: From 10 000 (1 liter of culture)
Purification of expressed proteins for further use
Description: Affinity chromatography, ion-exchange chromatography, gel permeation chromatography, etc.
Input: Protein, in the form of a soluble sample, cell lysate or cell pellet.
Output: Purified protein sample.
Time: Several days to several weeks (in case of optimizations).
Approximate price: From 8 000 (affinity chromatography), depending on the method
