Dynamic light scattering
Description: Offers measurement of the particle size distribution in solution, particle oligomerization, aggregation. Particle size range 1-1000 nm.
Input: Min. 1 μl of solution, min. concentration 0.5 mg/ml.
Output: Particle size distribution
Time: Next day
Approximate price: 1 000 (1 hour, ~ 6 samples per hour)
Small Angle X-ray Scattering (SAXS)
Description: In solution, it is possible to determine size of the particle, molecular weight, structural information at the level of particle shape (low resolution 3D structure), sample quality (aggregation). Analysis of mixture of components with known structure. The measurement can be coupled to liquid chromatography (SEC). Screening of various conditions is possible.
Input: Protein/DNA/RNA in monodisperse solution, concentration series 1-10 mg/ml, min. 3x 20 μl.
Output: Sample quality (aggregation), particle size and molecular weight estimate, ratio of components in the case of mixtures.
Time: Basic measurement and analysis - next day; advanced analysis - several days
Approximate price: From 2 000 to 5 000 per sample (depending on analysis level)
Differential scanning fluorimetry
Description: Characterization of protein thermostability using intrinsic tryptophan and tyrosine fluorescence to measure melting transitions.
Input: Min. 10 μl of protein solution, concentration from 0.1 mg/ml.
Output: Plot of melting transition, melting temperature
Time: Next day
Approximate price: From 300 per sample
Differential scanning calorimetry
Description: Characterization of thermostability of biopolymers.
Input: Min. 500 μl of biomolecule solution, concentration from 1 mg/ml. Output: Thermodynamic parameters of melting transition.
Time: Next day
Approximate price: From 5 000 per sample
