Laboratory of Reproductive Biology

Our laboratory has extensive knowledge of assessing male reproductive parameters as markers of fertility disorders. We focus on studying the molecular mechanisms of reproduction and the nature of specific sperm proteins that play a role in sperm maturation, sperm-egg interaction and early embryo development. The area of our interest covers monitoring of sperm quality in patients with testicular cancer and  characterisation of sperm antibodies in infertile couples. Our goal is to apply the knowledge of basic research into clinical practice, in order to develop new diagnostic tools for sperm quality assessment and gamete selection for Centres of Assisted Reproduction.

Recently, we have been studying in detail characterization of dynamic rearrangement of cytoskeletal proteins and their partner proteins (IZUMO1, CD46, CD9, CD81, CD151 and integrins) involved in sperm-egg fusion. In addition, the acrosin inhibitor expression and crucial ubiquitin proteasomal complex during sperm maturation has been addressed. Epigenetic aberrations (selected histone modifications and DNA methylation) in spermatozoa, testicular tissues and early embryos after exposure to environmental pollutants have been studied and new related project are in progress. All the ongoing research is supported by several prestigious grants. The outcome of this research is being translated into new diagnostic tools for identification of sperm parameters and selection of good and bad sperm to be used in Centres of Assisted Reproduction. This translational research is supported by grants of the Technological Agency of the Czech Republic and the Agency of Ministry of Health. Two European and two Czech patents were submitted.

We have productive collaboration based on the knowledge of the detailed assessment of reproductive parameters, monitoring of transgenerational epigenetic inheritance, characterization of epigenetic aberration and microRNA deregulations, all tailored for specific areas of interest (medical conditions, lifestyle, environmental factors). In addition, we utilize, in collaboration with commercial subjects, our knowledge of hybridoma technology for the production of specific monoclonal antibodies with high affinity, and twelve prototypes for the detection of relevant molecules have been developed. Moreover, we have valuable collaboration based on transgenic mouse lines, property of our laboratory, serving as indispensable tool for in vivo experiments, specifically transgenic C57BL/6^Acr3-EGFP mice expressing green protein (EGFP) in sperm acrosome, transgenic C57BL/6^Su9DsRed2 mice expressing red fluorescent protein (RFP) in somatic cell mitochondria.

ERN eUROGEN Webinar 79: Fertility issues in testicular cancer

This webinar was presented on Wednesday 19 July 2023 by Assoc. Prof. Katerina Komrskova, Head of Group Reproductive Biology, in the Institute of Biotechnology of the Czech Academy of Sciences.

Laboratory of Reproductive Biology in media

A fundamental step in human fertilisation has been discovered by researchers who say it may offer new insights into unexplained infertility. Read more details of this discovery in The Guardian magazine HERE.

2023

• Frolikova M, Sur VP, Novotny I, Blazikova M, Vondrakova J, Simonik O, Ded L, Valaskova E, Koptasikova L, Benda A, Postlerova P, Horvath O., Komrskova K (2023) "Juno and CD9 protein network organization in oolemma of mouse oocyte." Front. Cell Dev. Biol. 11:1110681. doi: 10.3389/fcell.2023.1110681

• Hackerova L, Klusackova B, Zigo M, Zelenkova N, Havlikova K, Krejcirova R, Sedmikova M, Sutovsky P, Komrskova K, Postlerova P and Simonik O (2023) "Modulatory effect of MG-132 proteasomal inhibition on
  boar sperm motility during in vitro capacitation."Front. Vet. Sci. 10:1116891. doi: 10.3389/fvets.2023.1116891

• Tepla, O., Topurko, Z., Jirsova, S., Moosova, M., Fajmonova, E., Cabela, R., Komrskova, K., Kratochvilova, I., Masata, J. (2023)"Timing of ICSI with Respect to Meiotic Spindle Int. J. Mol. Sci.https://doi.org/10.3390

• Otčenášková T, Macíčková E, Vondráková J, Frolíková M, Komrskova K, Stopková R, Stopka P. Proteomic analysis of the mouse sperm acrosome - towards an understanding of an organelle with diverse functionality. Eur J Cell Biol. 2023 Jun;102(2):151296. doi: 10.1016/j.ejcb.2023.151296

• Qasemi M, Sur VP, Simonik O, Postlerova P, Skrobanek P, Hradec T, Boublikova L, Zamecnik L, Buchler T, Neuzil J, Komrskova K. (2023) Sperm mitochondria dysfunction in response to testicular cancer. Eur J Clin Invest. e14146. doi: 10.1111/eci.14146. Epub ahead of print. PMID: 38069497.

2022

• Vondrakova, J., Frolikova, M. Ded, L., Cerny, J., Postlerova, P., Palenikova, V., Simonik, O., Nahacka, Z., Basus, K., Valaskova, E., Machan, R., Pacey, A., Holubcova, Z., Koubek, P., Ezrova, Z., Park, S., Liu, R., Partha, R., Clark, N., Neuzil, J., Ikawa, M., Erickson, K., Lam, K. S., Moore, H., Komrskova, K. (2022) „MAIA, Fc receptor–like 3, supersedes JUNO as IZUMO1 receptor during human fertilization“ Sci. Adv. 8, eabn0047 https://doi:10.1126/sciadv.abn0047

• Sur, V. P., Simonik, O., Novotna, M., Mazumdar, A., Liska, F., Vimberg, V., & Komrskova, K. (2022). "Dynamic study of small toxic hydrophobic proteins PepA1 and PepG1 of Staphylococcus aureus." Int J Biol Macromol. https://doi.org/10.1016/j.ijbiomac.2022.07.192

• Poignet, M., Barankova, L., Reif, J., Stopka, P., Stopková, R., Frolikova, M., Cramer, E.R.A., Johnsen, A., Kverek, P., Osiejuk, T.S., Komrskova, K., Albrecht, T., Reifova., R. (2022) "Sperm morphology and performance in relation to postmating prezygotic isolation in two recently diverged passerine species." Sci Rep 12, 22275 https://doi.org/10.1038/s41598-022-26101-5

• Mimouni, N. E. H., Ialy-Radio, c., Denizot, A.-L., Lagoutte, I., Frolikova, M., Komrskova, K., Barbaux, S., Ziyyat., A. (2022) „Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice“ Int. J. Mol. Sci. 23, 13812. https://doi.org/10.3390/ijms232213812

• de la Iglesia, A., Jauregi, P., Jodar, M., Barrachina, F., Ded, L., Mallofré, C., Rodríguez-Carunchio, L., Corral, J.M., Ballescà, J.L., Komrskova, K.,Castillo, J., Oliva, R. (2022) H4K5 „Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin“ Int. J. Mol. Sci. 23(12398). https://doi.org/10.3390/ijms232012398

• Antalikova, J., Secova, P., Michalkova, K., Horovska, L., Palenikova, V., & Jankovicova, J. (2022). "Expression of alphaV integrin and its potential partners in bull reproductive tissues, germ cells and spermatozoa." Int J Biol Macromol, 209(Pt A), 542-551. https://doi.org/10.1016/j.ijbiomac.2022.04.001

• Bosakova, T., Tockstein, A., Bosakova, Z., Komrskova, K. (2022) "Kinetic Study of 17 -Estradiol Mechanism during Rat Sperm Capacitation" Molecules 27 (4092) https://doi.org/10.3390/molecules27134092

• Simonik, O., Bubenickova, F., Tumova, L., Frolikova, M., Sur, V. P., Beran, J., Havlikova, K., Hackerova, L., Spevakova, Komrskova, K., Postlerova, P. "Boar Sperm Cryopreservation Improvement Using Semen Extender Modification by Dextran and Pentaisomaltose" (2022) Animals 12 (868) https://doi.org/10.3390/ani12070868

2021

• Levoux J, Prola A, Lafuste P, Gervais M, Chevallier N, Koumaiha Z, Kefi K, Braud L, Schmitt A, Yacia A, Schirmann A, Hersant B, Sid-Ahmed M, Ben Larbi S, Komrskova K, Rohlena J, Relaix F, Neuzil J, Rodriguez AM. (2021) "Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming." Cell Metab. 33(2):283-299. https://doi.org/10.1016/j.cmet.2021.02.003

• Sur, V.P., Mazumdar, A., Vimberg, V., Stefani, T, Androvic, L., Kracikova, L., Laga, R., Kamenik, Z., Komrskova, K. (2022) „Specific Inhibition of VanZ-Mediated Resistance to Lipoglycopeptide Antibiotics“ Int. J. Mol. Sci. 23(97). https://doi.org/10.3390/ijms23010097

• Sur, V. P., Sen, M.K., Komrskova, K. (2021) "In Silico Identification and Validation of Organic Triazole Based Ligands as Potential Inhibitory Drug Compounds of SARS-CoV-2 Main Protease" Molecules, 26 (6199( https://doi.org/10.3390/molecules26206199

• Bosakova, T., Tockstein, A., Bosakova, Z., Komrskova, K. "Kinetic Study of 17-Estradiol Activity in Comparison with 17-Estradiol and 17-Ethynylestradiol" (2021) Catalyst 11(634) https://doi.org/10.3390/catal11050634

• Kumar, R., Mazakova, J., Ali, A., Sur, V. P., Sen, M. K., Bolton, M. D., Manasova, M., Rysanek, P., & Zouhar, M. (2021, Dec 11). Characterization of the Molecular Mechanisms of Resistance against DMI Fungicides in Cercospora beticola Populations from the Czech Republic. J Fungi (Basel), 7(12). https://doi.org/10.3390/jof7121062

• Merc, V., Frolikova, M., Komrskova, K. (2021) "Role of Integrins in Sperm Activation and Fertilization"Int. J. Mol. Sci. 22 (11809) https://doi:10.3390/ijms222111809

• Navarro-Serna, S., Paris-Oller, E., Simonik, O., Romar, R., & Gadea, J. (2021). "Replacement of Albumin by Preovulatory Oviductal Fluid in Swim-Up Sperm Preparation Method Modifies Boar Sperm Parameters and Improves In Vitro Penetration of Oocytes." Animals (Basel), 11(5). https://doi.org/10.3390/ani11051202

• Palenikova, V., Frolikova, M., Valaskova, E., Postlerova, P., Komrskova, K. (2021)"αV Integrin Expression and Localization in Male Germ Cells" Int. J. Mol. Sci., 22(17), 9525, https://doi.org/10.3390/ijms22179525

• Tepla, O., Topurko, Z., Masata, J., Jirsova, S., Frolikova, M., Komrskova, K., Minks, A., Turanek, J., Lynnyk, A., Kratochvilova, I.(2021) "Important parameters affecting quality of vitrified donor oocytes" Cryobiology, https://doi.org/10.1016/j.cryobiol.2021.03.001

• Thomas, H.I.S., Chen, Y.-S., Hung, C.-H., Sreerangaraja Urs, D.B., Liao, T.-L., Lai, Y.-C., Komrskova, K., Postlerova, P., Lin, Y.-F., Kao, S.-H. (2021) "Genetic association in the maintenance of the mitochondrial microenvironment and sperm capacity" Oxi. Med. Cell. Long., https://doi.org/10.1155/2021/5561395

• Tumova, L., Zigo, M., Sutovsky, P., Sedmikova, M., Postlerova, P. (2021) "Ligands and Receptors Involved in the Sperm-Zona Pellucida Interactions in Mammals." Cells 10(1). https://doi.org/10.3390/cells10010133

Patents and utility models

• PCT/CZ2021/050004 and PCT/CZ2021/050005 applications in process
• PV 308863 and PV 308864
• Utility model no. 35085
• Certified methodology No. MZE-59116/2021-18141; ISBN: 978-80-213-3151-8

2020

• Levoux J, Prola A, Lafuste P, Gervais M, Chevallier N, Koumaiha Z, Kefi K, Braud L, Schmitt A, Yacia A, Schirmann A, Hersant B, Sid-Ahmed M, Ben Larbi S, Komrskova K, Rohlena J, Relaix F, Neuzil J, Rodriguez AM.(2020) "Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming." Cell Metab. S1550-4131(20)

• Ded, L., Hwang, J. Y., Miki, K., Shi, H. F., & Chung, J. J. (2020). "3D in situ imaging of female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice." Elife, 9.

• Jankovicova, J., M. Frolikova, V. Palenikova, E. Valaskova, J. Cerny, P. Secova, M. Bartokova, L. Horovska, P. Manaskova-Postlerova, J. Antalikova and K. Komrskova (2020). "Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells." Scientific Reports 10(1).

• Sreerangaraja Urs, D. B., W. H. Wu, K. Komrskova, P. Postlerova, Y. F. Lin, C. R. Tzeng and S. H. Kao (2020). "Mitochondrial Function in Modulating Human Granulosa Cell Steroidogenesis and Female Fertility." Int J Mol Sci 21(10).

• Frolikova, M., T. Otcenaskova, E. Valaskova, P. Postlerova, R. Stopkova, P. Stopka and K. Komrskova (2020). "The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes." Int J Mol Sci 21(7).

• Kmonickova V, Frolikova M, Steger K, Komrskova K. (2020) "The Role of the LINC Complex in Sperm Development and Function." Int J Mol Sci. 21(23).

• Antalikova J, Secova P, Horovska L, Krejcirova R, Simonik O, Jankovicova J, Bartokova M, Tumova L, Manaskova-Postlerova P. (2020) "Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?" Cells 9(1)

• Bosakova, T., A. Tockstein, N. Sebkova, R. Cabala and K. Komrskova (2020). "Kinetic Model of the Action of 17α-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS." Catalysts 10(1).

• Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. (2020) "Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation." Int J Mol Sci. 21(17):6415

• Dostalova, P., Zatecka, E., Ded, L., Elzeinova, F., Valaskova, E., Kubatova, A., Korenkova, V., Langerova, L., Komrskova, K., & Peknicova, J. (2020). "Gestational and pubertal exposure to low dose of di-(2-ethylhexyl) phthalate impairs sperm quality in adult mice." Reprod Toxicol, 96, 175-184.

• Hylmarova S, Stechova K, Pavlinkova G, Peknicova J, Macek M, Kvapil M. (2020) "The impact of type 1 diabetes mellitus on male sexual functions and sex hormone levels." Endocr J. 28;67(1)

• Jankovicova, J., Z. Neuerova, P. Secova, M. Bartokova, F. Bubenickova, K. Komrskova, P. Postlerova and J. Antalikova (2020). "Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos." Med Microbiol Immunol.

• Tumova, L., Zigo, M., Sutovsky, P., Sedmikova, M., & Postlerova, P. (2020). "The Ubiquitin-Proteasome System Does Not Regulate the Degradation of Porcine beta-Microseminoprotein during Sperm Capacitation." Int J Mol Sci 21(11).

• Zigo, M., P. Manaskova-Postlerova, D. Zuidema, K. Kerns, V. Jonakova, L. Tumova, F. Bubenickova and P. Sutovsky (2020). "Porcine model for the study of sperm capacitation, fertilization and male fertility." Cell Tissue Res 380(2): 237-262.

2019

• Baert, Y., K. Dvorakova-Hortova, H. Margaryan and E. Goossens (2019). "Mouse in vitro spermatogenesis on alginate-based 3D bioprinted scaffolds." Biofabrication 11(3).

• Bajzikova, M., J. Kovarova, A. R. Coelho, S. Boukalova, S. Oh, K. Rohlenova, D. Svec, S. Hubackova, B. Endaya, K. Judasova, A. Bezawork-Geleta, K. Kluckova, L. Chatre, R. Zobalova, A. Novakova, K. Vanova, Z. Ezrova, G. J. Maghzal, S. M. Novais, M. Olsinova, L. Krobova, Y. J. An, E. Davidova, Z. Nahacka, M. Sobol, T. Cunha-Oliveira, C. Sandoval-Acuna, H. Strnad, T. C. Zhang, T. Huynh, T. L. Serafim, P. Hozak, V. A. Sardao, W. J. H. Koopman, M. Ricchetti, P. J. Oliveira, F. Kolar, M. Kubista, J. Truksa, K. Dvorakova-Hortova, K. Pacak, R. Gurlich, R. Stocker, Y. Q. Zhou, M. V. Berridge, S. Park, L. F. Dong, J. Rohlena and J. Neuzil (2019). "Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells." Cell Metabolism 29(2): 399-+.

• Ded, L., P. Dostalova, E. Zatecka, A. Dorosh, K. Komrskova and J. Peknicova (2019). "Fluorescent analysis of boar sperm capacitation process in vitro." Reprod Biol Endocrinol 17(1): 109.

• Frolikova, M., E. Valaskova, J. Cerny, A. Lumeau, N. Sebkova, V. Palenikova, N. Sanches-Hernandez, A. Pohlova, P. Manaskova-Postlerova and K. Dvorakova-Hortova (2019). "Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm." Int J Mol Sci 20(5).

• Jankovicova, J., P. Secova, P. Manaskova-Postlerova, O. Simonik, M. Frolikova, E. Chmelikova, L. Horovska, K. Michalkova, K. Dvorakova-Hortova and J. Antalikova (2019). "Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos." Int J Biol Macromol 123: 931-938.

• Páleníková, V. and E. Tibenská (2019). "Význam a diagnostika antifosfolipidových protilátok u žien s poruchami reprodukcie." Newslab 10(1): 5.

• Simonik, O., J. Sichtar, J. Beran, P. Manaskova-Postlerova, L. Tumova, M. Dolezalova, P. Folkova, L. Stadnik and R. Rajmon (2019). "Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders." Animal Reproduction 16(2): 267-276.

• Zigo, M., P. Manaskova-Postlerova, V. Jonakova, K. Kerns and P. Sutovsky (2019). "Compartmentalization of the proteasome-interacting proteins during sperm capacitation." Scientific Reports 9.

• Zigo, M., V. Jonakova, P. Manaskova-Postlerova, K. Kerns and P. Sutovsky (2019). "Ubiquitin-proteasome system participates in the de-aggregation of spermadhesin and DQH protein during boar sperm capacitation." Reproduction.

2018

• Bosakova, T., A. Tockstein, N. Sebkova, O. Simonik, H. Adamusova, J. Albrechtova, T. Albrecht, Z. Bosakova and K. Dvorakova-Hortova (2018). "New Insight into Sperm Capacitation: A Novel Mechanism of 17beta-Estradiol Signalling." Int J Mol Sci 19(12).

• Frolikova, M., P. Manaskova-Postlerova, J. Cerny, J. Jankovicova, O. Simonik, A. Pohlova, P. Secova, J. Antalikova and K. Dvorakova-Hortova (2018). "CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization." Int J Mol Sci 19(4).

• Jankovicova, J., K. Michalkova, P. Secova, L. Horovska, P. Manaskova-Postlerova and J. Antalikova (2018). "Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis." Cell Tissue Res 371(2): 365-373.

• Kerns, K., M. Zigo, E. Z. Drobnis, M. Sutovsky and P. Sutovsky (2018). "Zinc ion flux during mammalian sperm capacitation." Nature Communications 9(1): 2061.

• Krejcirova, R., M. Manasova, V. Sommerova, E. Langhamerova, R. Rajmon and P. Manaskova-Postlerova (2018). "G protein-coupled estrogen receptor (GPER) in adult boar testes, epididymis and spermatozoa during epididymal maturation." Int J Biol Macromol 116: 113-119.

• Zigo, M., K. Kerns, M. Sutovsky and P. Sutovsky (2018). "Modifications of the 26S proteasome during boar sperm capacitation." Cell Tissue Res 372(3): 591-601.

2017

• Dong, L. F., J. Kovarova, M. Bajzikova, A. Bezawork-Geleta, D. Svec, B. Endaya, K. Sachaphibulkij, A. R. Coelho, N. Sebkova, A. Ruzickova, A. S. Tan, K. Kluckova, K. Judasova, K. Zamecnikova, Z. Rychtarcikova, V. Gopalan, L. Andera, M. Sobol, B. Yan, B. Pattnaik, N. Bhatraju, J. Truksa, P. Stopka, P. Hozak, A. K. Lam, R. Sedlacek, P. J. Oliveira, M. Kubista, A. Agrawal, K. Dvorakova-Hortova, J. Rohlena, M. V. Berridge and J. Neuzil (2017). "Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells." Elife 6.

• Dostalova, P., E. Zatecka and K. Dvorakova-Hortova (2017). "Of Oestrogens and Sperm: A Review of the Roles of Oestrogens and Oestrogen Receptors in Male Reproduction." Int J Mol Sci 18(5).

• Drab, T., S. Ren, P. Manaskova-Postlerova, M. Ticha, V. Jonakova and J. Liberda (2017). "Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction." Theriogenology 100: 80-87.

• Pavlinkova, G., H. Margaryan, E. Zatecka, E. Valaskova, F. Elzeinova, A. Kubatova, R. Bohuslavova and J. Peknicova (2017). "Transgenerational inheritance of susceptibility to diabetes-induced male subfertility." Sci Rep 7(1): 4940.

2016

• Bosakova, Z., A. Tockstein, H. Adamusova, P. Coufal, N. Sebkova and K. Dvorakova-Hortova (2016). "Kinetic analysis of decreased sperm fertilizing ability by fluorides and fluoroaluminates: a tool for analyzing the effect of environmental substances on biological events." Eur Biophys J 45(1): 71-79.

• Capkova, J., A. Kubatova, L. Ded, O. Tepla and J. Peknicova (2016). "Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies." Asian J Androl 18(1): 108-113.

• Frolikova, M., N. Sebkova, L. Ded and K. Dvorakova-Hortova (2016). "Characterization of CD46 and beta1 integrin dynamics during sperm acrosome reaction." Sci Rep 6: 33714.

• Jankovicova, J., M. Frolikova, N. Sebkova, M. Simon, P. Cupperova, D. Lipcseyova, K. Michalkova, L. Horovska, R. Sedlacek, P. Stopka, J. Antalikova and K. Dvorakova-Hortova (2016). "Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes." Reproduction 152(6): 785-793.

• Manaskova-Postlerova, P., N. Cozlova, A. Dorosh, M. Sulc, B. Guyonnet and V. Jonakova (2016). "Acrosin inhibitor detection along the boar epididymis." Int J Biol Macromol 82: 733-739.

• Pěknicová, J., Ulčová-Gallová, Z., Madar, J. (2016). Imunologie a imunopatologie mužského pohlavního systému. [Immunology and immunopathology of the male reproductive system.]. Imunologie a imunopatologie lidské reprodukce. Praha, Mladá fronta: 22-29.

• Pěknicová, J., Ulčová-Gallová, Z., Madar, J. (2016). Imunologie oplození.  [Immunology of fertilization.]. Imunologie a imunopatologie lidské reprodukce. Praha, Mladá fronta. 5: 39-51.

2015

• Brieno-Enriquez, M. A., J. Garcia-Lopez, D. B. Cardenas, S. Guibert, E. Cleroux, L. Ded, D. Hourcade Jde, J. Peknicova, M. Weber and J. Del Mazo (2015). "Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells." Plos One 10(4): e0124296.

• Capkova, J., H. Margaryan, A. Kubatova, P. Novak and J. Peknicova (2015). "Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men." Basic Clin Androl 25: 11.

• Margaryan, H., A. Dorosh, J. Capkova, P. Manaskova-Postlerova, A. Philimonenko, P. Hozak and J. Peknicova (2015). "Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm." Reprod Biol Endocrinol 13: 15.

• Vieweg, M., K. Dvorakova-Hortova, B. Dudkova, P. Waliszewski, M. Otte, B. Oels, A. Hajimohammad, H. Turley, M. Schorsch, H. C. Schuppe, W. Weidner, K. Steger and A. Paradowska-Dogan (2015). "Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients." Clin Epigenetics 7: 31.

• Zigo, M., A. Dorosh, A. Pohlova, V. Jonakova, M. Sulc and P. Manaskova-Postlerova (2015). "Panel of monoclonal antibodies to sperm surface proteins as a tool for monitoring localization and identification of sperm-zona pellucida receptors." Cell Tissue Res 359(3): 895-908.

2014

• Alavi, S. M., P. Postlerova-Manaskova, A. Hatef, M. Psenicka, J. Peknicova, K. Inaba, A. Ciereszko and O. Linhart (2014). "Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity." Fish Physiol Biochem 40(5): 1393-1398.

• Dvorakova-Hortova, K., A. Sidlova, L. Ded, D. Hladovcova, M. Vieweg, W. Weidner, K. Steger, P. Stopka and A. Paradowska-Dogan (2014). "Toxoplasma gondii decreases the reproductive fitness in mice." Plos One 9(6): e96770.

• Sebkova, N., L. Ded, K. Vesela and K. Dvorakova-Hortova (2014). "Progress of sperm IZUMO1 relocation during spontaneous acrosome reaction." Reproduction 147(2): 231-240.

• Zatecka, E., J. Castillo, F. Elzeinova, A. Kubatova, L. Ded, J. Peknicova and R. Oliva (2014). "The effect of tetrabromobisphenol A on protamine content and DNA integrity in mouse spermatozoa." Andrology 2(6): 910-917.

• Zatecka, E., L. Ded, F. Elzeinova, A. Kubatova, A. Dorosh, H. Margaryan, P. Dostalova, V. Korenkova, K. Hoskova and J. Peknicova (2014). "Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice." Reprod Toxicol 45: 20-30.

2013

• Ded, L., N. Sebkova, M. Cerna, F. Elzeinova, P. Dostalova, J. Peknicova and K. Dvorakova-Hortova (2013). "In vivo exposure to 17beta-estradiol triggers premature sperm capacitation in cauda epididymis." Reproduction 145(3): 255-263.

• Dorosh, A., O. Tepla, E. Zatecka, L. Ded, K. Koci and J. Peknicova (2013). "Expression analysis of MND1/GAJ, SPATA22, GAPDHS and ACR genes in testicular biopsies from non-obstructive azoospermia (NOA) patients." Reprod Biol Endocrinol 11: 42.

• Elzeinova, F., J. Peknicova, L. Ded, A. Kubatova, H. Margaryan, A. Dorosh, P. Makovicky and R. Rajmon (2013). "Adverse effect of tetracycline and doxycycline on testicular tissue and sperm parameters in CD1 outbred mice." Exp Toxicol Pathol 65(6): 911-917.

• Zatecka, E., L. Ded, F. Elzeinova, A. Kubatova, A. Dorosh, H. Margaryan, P. Dostalova and J. Peknicova (2013). "Effect of tetrabrombisphenol A on induction of apoptosis in the testes and changes in expression of selected testicular genes in CD1 mice." Reprod Toxicol 35: 32-39.

• Zigo, M., V. Jonakova, M. Sulc and P. Manaskova-Postlerova (2013). "Characterization of sperm surface protein patterns of ejaculated and capacitated boar sperm, with the detection of ZP binding candidates." Int J Biol Macromol 61: 322-328.

2012

• Capkova, J., T. Hrncir, A. Kubatova and H. Tlaskalova-Hogenova (2012). "Lipopolysaccharide treatment suppresses spontaneously developing ankylosing enthesopathy in B10.BR male mice: the potential role of interleukin-10." BMC Musculoskelet Disord 13: 110.

• Sebkova, N., M. Cerna, L. Ded, J. Peknicova and K. Dvorakova-Hortova (2012). "The slower the better: how sperm capacitation and acrosome reaction is modified in the presence of estrogens." Reproduction 143(3): 297-307.

• Yi, Y. J., S. W. Zimmerman, G. Manandhar, J. F. Odhiambo, C. Kennedy, V. Jonakova, P. Manaskova-Postlerova, M. Sutovsky, C. S. Park and P. Sutovsky (2012). "Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization." Int J Androl 35(2): 196-210.

2011

• Dorosh, A., L. Ded, F. Elzeinova and J. Peknicova (2011). "Assessing oestrogenic effects of brominated flame retardants hexabromocyclododecane and tetrabromobisphenol A on MCF-7 cells." Folia Biol (Praha) 57(1): 35-39.

• Dorosh, A. (2011). "Monoclonal Antibody 6E4 Against Human GAPDHS Protein." Hybridoma 30(3): 321-321.

• Drab, T., J. Kracmerova, I. Ticha, E. Hanzlikova, M. Ticha, H. Ryslava, V. Doubnerova, P. Manaskova-Postlerova and J. Liberda (2011). "Native red electrophoresis--a new method suitable for separation of native proteins." Electrophoresis 32(24): 3597-3599.

• Manaskova-Postlerova, P., N. Davidova, M. Sulc, A. Philimonenko, P. Hozak and V. Jonakova (2011). "Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein." Cell Tissue Res 344(2): 341-353.

• Manaskova-Postlerova, P., N. Davidova and V. Jonakova (2011). "Biochemical and binding characteristics of boar epididymal fluid proteins." J Chromatogr B Analyt Technol Biomed Life Sci 879(1): 100-106.

• Uhnakova, B., R. Ludwig, J. Peknicova, L. Homolka, L. Lisa, M. Sulc, A. Petrickova, F. Elzeinova, H. Pelantova, D. Monti, V. Kren, D. Haltrich and L. Martinkova (2011). "Biodegradation of tetrabromobisphenol A by oxidases in basidiomycetous fungi and estrogenic activity of the biotransformation products." Bioresour Technol 102(20): 9409-9415.

• Zigo, M., V. Jonakova and P. Manaskova-Postlerova (2011). "Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms." Electrophoresis 32(11): 1309-1318.

2010

• Ded, L., P. Dostalova, A. Dorosh, K. Dvorakova-Hortova and J. Peknicova (2010). "Effect of estrogens on boar sperm capacitation in vitro." Reprod Biol Endocrinol 8: 87.

• Li, P., M. Hulak, P. Koubek, M. Sulc, B. Dzyuba, S. Boryshpolets, M. Rodina, D. Gela, P. Manaskova-Postlerova, J. Peknicova and O. Linhart (2010). "Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L." Theriogenology 74(3): 413-423.

• Yi, Y. J., G. Manandhar, M. Sutovsky, S. W. Zimmerman, V. Jonakova, F. W. van Leeuwen, R. Oko, C. S. Park and P. Sutovsky (2010). "Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization." Cell Tissue Res 341(2): 325-340.

• Yi, Y. J., G. Manandhar, M. Sutovsky, V. Jonakova, C. S. Park and P. Sutovsky (2010). "Inhibition of 19S proteasomal regulatory complex subunit PSMD8 increases polyspermy during porcine fertilization in vitro." J Reprod Immunol 84(2): 154-163.

2009

• Davidova, N., V. Jonakova and P. Manaskova-Postlerova (2009). "Expression and localization of acrosin inhibitor in boar reproductive tract." Cell Tissue Res 338(2): 303-311.

• Moos, J., K. Rezabek, V. Filova, M. Moosova, J. Pavelkova and J. Peknicova (2009). "Comparison of follicular fluid and serum levels of Inhibin A and Inhibin B with calculated indices used as predictive markers of Ovarian Hyperstimulation Syndrome in IVF patients." Reprod Biol Endocrinol 7: 86.

2008

• Elzeinova, F., V. Novakova, D. Buckiova, A. Kubatova and J. Peknicova (2008). "Effect of low dose of vinclozolin on reproductive tract development and sperm parameters in CD1 outbred mice." Reprod Toxicol 26(3-4): 231-238.

• Koubek, P., A. Kralova, M. Psenicka and J. Peknicova (2008). "The optimization of the protocol for immunofluorescence on fish spermatozoa." Theriogenology 70(5): 852-858.

• Kralova, A., M. Svetlikova, J. Madar, Z. Ulcova-Gallova, A. Bukovsky and J. Peknicova (2008). "Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study." Reprod Biol Endocrinol 6: 27.

• Sinkorova, Z., J. Capkova, J. Niederlova, R. Stepankova and J. Sinkora (2008). "Commensal intestinal bacterial strains trigger ankylosing enthesopathy of the ankle in inbred B10.BR (H-2(k)) male mice." Hum Immunol 69(12): 845-850.

GAČR: GA23-06591S, L. Děd: 3D in situ molecular analysis of the reproductive processes in the natural and pathological states. 2023 – 2025

GAČR: GF23-07028K, K. Komrsková: Breaking the rules: modification of sexual reproduction pathways in vertebrates. 2023 - 2026

GAČR: GA-22-30494S, Komrsková: The role of FcRL1 and FcRL3 proteins during gamete membrane binding and fusion. Grant Agency of the Czech Republic. 2022 - 2024

GAČR: GA-22-31156S, Postlerová: Key molecules involved in gamete maturation and sperm-zona pellucida recognition in pig and cattle. 2022 - 2024

COST: OC-2020-1-24708, Komrsková: ANDRONET (European andrology network– research coordination, education and public awareness).

AZV: NU20-03-00309, Komrsková: Monitoring of fertility and testicular tissue status in testicular cancer patients depending on their therapy. 2020-2023.

GAČR: GJ20-17403Y, Děd: Effect of Estrogens on Testicular/Sperm Epigenome and Early Embryonic Development. 2020-2022.

GAČR: GC20-20217J, Komrsková: The sperm LINC complex: The LINK between chromatin integrity, sperm motility and male fertility. 2020-2022.

TAČR Zéta: TJ02000219, Frolíková (PI); Komrsková (mentor): Novel tools for diagnostic and improvement of fertilization ability of sperm. 2019-2021.

AVČR (bilaterální dohoda se SAV): SAV-18-17, Postlerová: Study of molecules involved in the fertilization process of mammals. 2018-2020.

GAČR: GA18-11275S, Hortová: Monitoring of protein network dynamics during sperm-egg membrane interaction. 2018-2020.

GAČR: GA18-14325S, Hortová: The genetic basis of species origin: What can we learn from organisms with female heterogamety? 2018-2020.

AZV: NV15-30880A, Pěknicová: Proteomic and genetic assessment of sperm quality for the enhancement of assisted reproduction in infertile patients with diabetes mellitus. 2015-2018.

GAČR GA14-05547S Hortová: Molecular mechanisms involved in mammalian sperm capacitation and acrosome reaction 2014 - 2016

AVČR (bilaterální dohoda se SAV): SAV-15-05, Postlerová: Study of CD molecules on mammalian sperm. 2015-2017.

GAČR: GA14-05547S, Jonáková: Molecular mechanisms involved in mammalian sperm capacitation and acrosome reaction. 2014-2016.

AVČR (bilaterální dohoda s BAS): Expression genes and proteins with role in the fertilization. 2014-2016.

GAČR: GAP503/12/1834, Pěknicová: Identification of epigenetic biomarkers of male germ cell disorders linked to adverse environmental factors. 2012-2015.

GAUK: 618812 Identification and characterization of estrogen receptor variants in testes and sperm. 2012-2014.

GAČR: GA523/09/1793, Pěknicová: Vliv endokrinních disruptorů na reprodukční parametry a expresi vybraných genů v myších a rybích gonádách. 2009-2012.

GAČR: GA303/09/1285, Postlerová: Study of the sperm membrane proteins with zona pellucida binding activity. 2009-2011.

MZ: NS10009, Pěknicová: Expression of genes and proteins playing a role in the human reproduction - damage biomarkers. 2009-2011.

GAČR: GD523/08/H064, Biotechnologies of mammalian gametes. 2008-2011.

MEYS: 1M06011, The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment., 2006 - 2011

GAČR: GA524/06/0817, Ultrastructure, Energetic and Competition in Spermatozoa: A Comparative Study Using Two Model Species of Chondrostean and Teleostean Fishes. 2006-2008.

GAČR: GA303/06/0895, Jonáková: Characterization of proteins secreted by the male reproductive tract and their role in individual steps of the reproduction process. 2006-2008.

MZ: NR8932, Differential expression of Pregnancy Associated Plasma Protein - A (PAPP-A) and Insulin-like growth factor-binding protein 4 (IGFBP4) in follicular fluid and blood of women in correlation with ovarian hyperstimulation syndrome (OHSS). 2006-2008.

MŠMT: Eureka projekt: OE 211, ELISA kits to detect environmental quality. 2006-2008.

MŠMT: Národní program výzkumu II: 2B06151, Pěknicová: Biodegradation of polybrominated compounds, monitoring changes in the concentration of target pollutants and degradation intermediates in the environment. 2006-2010.

MŠMT: 1M06011, Pěknicová: Center for Molecular Methods of Monitoring Diffuse Environmental Pollution. 2006-2012.