Laboratory of Structural Proteins

Cytoskeletal dynamics at single molecule resolution

Group leader: RNDr. Zdeněk Lánský, Ph.D.

Cytoskeletal networks form the internal dynamic scaffold of living cells essential for key cellular processes, such as cell division, cell motility or morphogenesis. Ensembles of cytoskeletal proteins self-assemble to drive these processes. Our aim is to understand the principles that underpin their collective action resulting in the generation of a coherent behavior of the cytoskeletal networks.

We study i) neuronal pathfinding, a foundational process in ontogenetic development, ii) contractility of actin networks and their anchoring to the plasma membrane, key for cytokinesis, the final stage of cell division, iii) regulatory roles of intrinsically disordered, microtubule-associated proteins, essential axonal factors known for their roles in a number of neurodegenerative diseases and iv) long-range intracellular transport and trafficking of organelles, key for example for the maintenance of neuronal function. 

Our main experimental strategy is bottom-up reconstitution of cytoskeletal networks from individual components in vitro. We use genetic manipulations, biochemical and biophysical methods and mathematical modelling. Central to our approach are imaging and force measurement techniques with single molecule resolution. Employing methods of physics, we quantitatively describe the studied biological systems and predict their behavior.

Beber Alexandre
Braun Marcus
Čermáková Nikola, Mgr., Ph.D.
Gryčová Lenka, Mgr., Ph.D.
Karhanová Adéla
Khuntsariya Daria, Ing.
Krattenmacher Jochen
Lánský Zdeněk, RNDr., Ph.D.
Podhájecký Roman
Prakash Manjari
Sabó Ján, Mgr.
Siahaan Valerie
Šliková Pavlína
Váňová Veronika, Mgr.
Zdobinský Tomáš, Mgr.
Žatecká Eva, Mgr., Ph.D.

2021

  • Vala, M., Bujak, Ł., García Marín, A., Holanová, K., Henrichs, V., Braun, M., Lánský, Z., Piliarik, M., Nanoscopic "Structural Fluctuations of Disassembling Microtubules Revealed by Label‐Free Super‐Resolution Microscopy." Small Methods, 2000985

2020

  • Braun M, Diez S, Lansky Z. (2020) "Cytoskeletal organization through multivalent interactions." J Cell Sci, 133(12)

  • Braun M, Lansky Z. (2020) "Membrane Remodeling: Passive Crosslinkers Drive Membrane Tubulation." Current Biology, 30(6):R270-R272
  • Henrichs, V., Grycova, L., Barinka, C., Nahacka, Z., Neuzil, J., Diez, S., Rohlena, J., Braun, M., & Lansky, Z. (2020). "Mitochondria-adaptor TRAK1 promotes kinesin-1 driven transport in crowded environments." Nature Communications, 11(1), 3123.
  • Horníková L, Bruštíková K, Ryabchenko B, Zhernov I, Fraiberk M, Mariničová Z, Lánský Z, Forstová J."The Major Capsid Protein, VP1, of the Mouse Polyomavirus Stimulates the Activity of Tubulin Acetyltransferase 1 by Microtubule Stabilization." Viruses, 2020, 12(2):227
  • Monzon, G. A., Scharrel, L., A, D. S., Henrichs, V., Santen, L., & Diez, S. (2020, Nov 30). Stable tug-of-war between kinesin-1 and cytoplasmic dynein upon different ATP and roadblock concentrations. J Cell Sci, 133(22). https://doi.org/10.1242/jcs.249938
  • Zhernov, I., Diez, S., Braun, M., Lansky, Z. (2020). "Intrinsically Disordered Domain of Kinesin-3 Kif14 Enables Unique Functional Diversity." Curr Biol, 30(17), 3342-3351 e3345.

2019

  • Schmidt-Cernohorska, M., I. Zhernov, E. Steib, M. Le Guennec, R. Achek, S. Borgers, D. Demurtas, L. Mouawad, Z. Lansky, V. Hamel and P. Guichard (2019). "Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails." Science 363(6424): 285-288.

  • Siahaan, V., J. Krattenmacher, A. A. Hyman, S. Diez, A. Hernandez-Vega, Z. Lansky and M. Braun (2019). "Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes." Nat Cell Biol 21(9): 1086-1092.

2018

  • Ludecke, A., A. M. Seidel, M. Braun, Z. Lansky and S. Diez (2018). "Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules." Nature Communications 9(1): 2214.

2017

  • Braun, M., Z. Lansky, A. Szuba, F. W. Schwarz, A. Mitra, M. Gao, A. Ludecke, P. R. Ten Wolde and S. Diez (2017). "Changes in microtubule overlap length regulate kinesin-14-driven microtubule sliding." Nat Chem Biol 13(12): 1245-1252.

  • Havelka, D., M. A. Deriu, M. Cifra and O. Kucera (2017). "Deformation pattern in vibrating microtubule: Structural mechanics study based on an atomistic approach." Sci Rep 7(1): 4227.

  • Hernandez-Vega, A., M. Braun, L. Scharrel, M. Jahnel, S. Wegmann, B. T. Hyman, S. Alberti, S. Diez and A. A. Hyman (2017). "Local Nucleation of Microtubule Bundles through Tubulin Concentration into a Condensed Tau Phase." Cell Rep 20(10): 2304-2312.

  • Skultetyova, L., K. Ustinova, Z. Kutil, Z. Novakova, J. Pavlicek, J. Mikesova, D. Trapl, P. Baranova, B. Havlinova, M. Hubalek, Z. Lansky and C. Barinka (2017). "Human histone deacetylase 6 shows strong preference for tubulin dimers over assembled microtubules." Sci Rep 7(1): 11547.

2016

  • Braun, M., S. Diez and Z. Lansky (2016). "Cell Biology: Kinesin-14 Backsteps to Organize Polymerizing Microtubules." Curr Biol 26(24): R1292-R1294.

  • Braun, M., Z. Lansky, F. Hilitski, Z. Dogic and S. Diez (2016). "Entropic forces drive contraction of cytoskeletal networks." Bioessays 38(5): 474-481.

  • Jirku, M., Z. Lansky, L. Bednarova, M. Sulc, L. Monincova, P. Majer, L. Vyklicky, J. Vondrasek, J. Teisinger and K. Bousova (2016). "The characterization of a novel S100A1 binding site in the N-terminus of TRPM1." Int J Biochem Cell Biol 78: 186-193.

2015

  • Grycova, L., B. Holendova, Z. Lansky, L. Bumba, M. Jirku, K. Bousova and J. Teisinger (2015). "Ca(2+) binding protein S100A1 competes with calmodulin and PIP2 for binding site on the C-terminus of the TPRV1 receptor." ACS Chem Neurosci 6(3): 386-392.

GACR: GA20-04068S, Braun: Translating microtubule envelope sectioning into cytoskeletal structure and function. 2020-2022.

GACR: GX19-27477X, Lánský: Cytoskeletal mechanics of the growth cone steering. 2019-2023.

GACR: GA18-08304S, Lánský: Reconstitution of mitochondrial trasport system in vitro. 2018-2020.

GACR: GA18-19705S, Lánský: High-fidelity fast tracking of protein motion mechanisms. 2018-2020.

GACR: GJ17-12496Y, Braun: The role of protein friction in the adaptive regulation of microtubule depolymerization and rescue. 2017-2019.

GACR: GA15-17488S, Lánský: Force coupling between microtubules and actin filaments by unconventional myosins. 2015-2017.

MEYS (OP RDI): ED2.1.00/19.0390, Lánský: Introduction of new research methods to BIOCEV. 2015.